Effects of substitution of tryptophan 412 in the substrate activation pathway of yeast pyruvate decarboxylase.


Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate decarboxylase (EC from Saccharomyces cerevisiae at W412, located on the putative substrate activation pathway and linking E91 on the alpha domain with W412 on the gamma domain of the enzyme. While C221 on the beta domain is the residue at which substrate activation is… (More)


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