Effects of storage temperature and time on clinical biochemical parameters from rat serum.


Serum is often frozen and banked for analysis at a later date. This study assessed the stability of 17 analytes in rat serum during refrigeration at 4 degrees C and extended storage at -20 degrees C (frost-free and nonfrost-free freezers) and -70 degrees C. Samples were analyzed by using an automated dry-slide chemistry analyzer at time 0 and then stored as aliquots for analysis at time points including day 7, 30, 90, and 360. After 7 d of refrigeration, only creatine kinase activity had varied by more than 10% of the starting value. Freezing at -70 degrees C was clearly superior to -20 degrees C where changes were observed in CO(2) as early as day 30 and alanine aminotransferase as early as day 90. Samples stored in frost-free and nonfrost-free -20 degrees C freezers did not differ significantly through day 90. Factors such as storage time and temperature should be considered when designing any retrospective study.

Citations per Year

59 Citations

Semantic Scholar estimates that this publication has 59 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Cray2009EffectsOS, title={Effects of storage temperature and time on clinical biochemical parameters from rat serum.}, author={Carolyn Cray and Marilyn Rodriguez and Julia Zaias and Norman H. Altman}, journal={Journal of the American Association for Laboratory Animal Science : JAALAS}, year={2009}, volume={48 2}, pages={202-4} }