Effects of stimulation on steroid output and perfusion medium flow rate in the isolated perfused rat adrenal gland in situ.

  title={Effects of stimulation on steroid output and perfusion medium flow rate in the isolated perfused rat adrenal gland in situ.},
  author={Joy P. Hinson and Gavin Paul Vinson and BARBARA J. Whitehouse and Gavrielle M. Price},
  journal={The Journal of endocrinology},
  volume={109 2},
Using the in-situ, isolated, perfused rat adrenal system, the actions of adrenal stimulants on steroidogenesis and perfusion medium flow rates (under constant perfusion pump conditions) have been studied. In a series of 100 experiments, initial rates of corticosterone output and flow rates were found to be positively correlated, although there was no such relationship between initial rates of aldosterone output and flow rates. Furthermore, in stable perfusion conditions, bolus injections of… 
Regulation of adrenal vascular tone: role of endothelin-1 and nitric oxide.
The results suggest that endogenous endothelin causes a tonic vasoconstriction in the rat adrenal gland, mediated by the ETA receptor and that endogenous NO exerts a tonics vasodilatory effect on theRat adrenal.
Steroid-producing cells regulate arterial tone of adrenal cortical arteries.
It is concluded that ZG cells release EETs and this release is stimulated by ACTH, which represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow.
Indirect evidence for short-loop negative feedback of insulin secretion in the rat.
The data support a negative feedback inhibition of insulin secretion by secreted insulin, since insulin secretion was decreased by either adding exogenous insulin or by lowering endogenous insulin as the consequence of increased flow rates.
Vascularization of the adrenal cortex: its possible involvement in the regulation of steroid hormone release
It is postulated that regulation of venous drainage, via the vascular dam, plays an important role in the storage of the secretory product during the animals' inactive phase, and in the initial rapid rise in plasma levels of the glucocorticoids seen in response to stress or injection of ACTH.
Aldosterone secretagogues increase adrenal blood flow in male rats.
The results demonstrate that Ang II and ACTH increases in ABF are mediated by a cytochrome P450 metabolite, and laser-Doppler flowmetry is a useful tool for understanding ABF regulation by peptides that stimulate steroid hormone release.
Adrenocorticotropin causes vasodilatation in the human fetal-placental circulation.
It is demonstrated that ACTH causes a reduction in fetal placental vascular resistance in the human fetal-placental circulation in vitro, and neither nitric oxide nor PG-mediated pathways appear to be involved.


Control of zona glomerulosa function in the isolated perfused rat adrenal gland in situ.
The amount of aldosterone produced by this preparation was much higher than the amount produced by dispersed cell preparations, and closely approximated to the levels of a Aldosterone obtained in adrenal vein blood.
Studies on the mechanism of secretion of corticosteroids by the isolated perfused adrenal of the rat.
The results are consistent with those obtained in previous in-vitro studies and have been interpreted as suggesting that the main mechanism of corticosterone secretion is simple diffusion, while 18-hydroxy-DOC secretion, at least at sub-maximal levels of stimulation, appears to require a more complex process.
Adrenal blood flow and the adrenocortical response to corticotropin.
  • J. Urquhart
  • Biology, Medicine
    The American journal of physiology
  • 1965
It is concluded that adrenocortical function is an equivocal index of ACTH release by the pituitary, unless adrenoc Cortical blood flow is known to be constant.
Corticosterone secretion in rats as a function of ACTH input and adrenal blood flow.
The rate of secretion of corticosterone from the left adrenal of rats receiving a constant input of ACTH was determined for different flows of blood through the adrenal during the 2- to 3-hr interval
The relationship between tissue preparation and function; methods for the study of control of aldosterone secretion: A review
While corticosterone is a major product of glomerulosa tissue in vitro, the available evidence suggests that it is not a major glomersulosa product in vivo.
Effects of DDD on Steroid Response and Blood Flow Through the Adrenal After ACTH.∗
  • J. Nichols, A. W. Richardson
  • Medicine, Biology
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1960
Conclusions Technical DDD fed to dogs in 200 mg/kg doses once daily for 3 days resulted in histologically normal adrenal glands which responded to ACTH with the expected increase in blood flow, but
Blood flow through the adrenal gland.
The blood flow through carotid jugular adrenal autotransplants has been studied in conscious sheep and shows no consistent relationship to the rate of output of any of the steroids, when these vary primarily.
It is concluded that prostaglandins are not required for steroidogenesis or the adrenal haemodynamic response to ACTH, and the response of adrenal blood flow to ACTH was measured in anaesthetized, dexamethasone‐treated, mongrel dogs.
Perfusion of the isolated adrenals in situ.
It was found that unless both recipient and donor dogs are hypophysectomized, the adrenal glands of the recipient dog are maximally stimulated and are not responsive to large doses of ACTH.
Adrenal blood flow response to adrenocorticotrophic hormone and other stimuli in the dog
The rubidium86 method yielded the same results in dogs as those obtained by Sapirstein in rats after ACTH, and hypotension induced by bleeding and vasoconstriction elicited with vasopressin produced less substantial reductions in adrenal than in renal and thyroidal blood flow.