Effects of sodium butyrate on human colonic adenocarcinoma cells. Induction of placental-like alkaline phosphatase.

@article{Gum1987EffectsOS,
  title={Effects of sodium butyrate on human colonic adenocarcinoma cells. Induction of placental-like alkaline phosphatase.},
  author={James R. Gum and Wing Kam and James C. Byrd and James W Hicks and Marvin H. Sleisenger and Yeong Sang Kim},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 3},
  pages={1092-7}
}
We have examined the effects of the "differentiating agent," sodium butyrate, on the induction of alkaline phosphatase in human colonic tumor cell line LS174T. Culture of these cells in the presence of 2 mM butyrate caused this activity to increase from less than 0.0001 unit/mg of protein to greater than 0.7 unit/mg of protein over an 8-day period. This induction proceeded in a nonlinear fashion with a lag time of 2-3 days occurring before enzymatic activity began to rise. These increases in… CONTINUE READING

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