Effects of sodium and GTP on the binding kinetics of [3H]diprenorphine in NG 108-15 cell membranes


Equilibrium binding isotherms of [3H]diprenorphine in membranes from NG 108-15 cells are consistent with a homogenous population of binding sites. Upon addition of Na+, Mg2+ and GTP, only a 2-fold reduction in affinity with a minor decrease in the number of sites is observed. Dissociation curves of [3H]diprenorphine, however, are clearly biphasic: in the… (More)
DOI: 10.1007/BF00569384


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