Effects of serum from patients with acute liver failure due to paracetamol overdose on human hepatocytes in vitro.

@article{Mitry2005EffectsOS,
  title={Effects of serum from patients with acute liver failure due to paracetamol overdose on human hepatocytes in vitro.},
  author={Ragai R. Mitry and R. D. Hughes and Sanjay Bansal and Sharon C. Lehec and Julia A. Wendon and Anil Dhawan},
  journal={Transplantation proceedings},
  year={2005},
  volume={37 5},
  pages={2391-4}
}
OBJECTIVE Our goal was to investigate the effects of serum from patients with acute liver failure due to paracetamol (acetaminophen) overdose on the function of human hepatocytes in vitro. METHODS Freshly isolated human hepatocytes plated on collagen-coated culture plates were, incubated (24 hours 37 degrees C) in medium containing pooled human sera (0%-80%) obtained from normal individuals or from patients with acute liver failure due to paracetamol overdose. The effects of the sera on cell… CONTINUE READING