Effects of serum-free culture media on in vitro development of domestic cat embryos following in vitro maturation and fertilization.

@article{Murakami2002EffectsOS,
  title={Effects of serum-free culture media on in vitro development of domestic cat embryos following in vitro maturation and fertilization.},
  author={M K Murakami and Takeshige Otoi and Ni Wayan Kurniani Karja and Andreas Ooka and Tatsuyuki Suzuki},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2002},
  volume={37 6},
  pages={352-6}
}
This study was conducted to determine the adequate medium for a serum-free culture system of domestic cat embryos produced by in vitro maturation (IVM) and fertilization (IVF). Cumulusoocyte complexes recovered from cat ovaries were matured in vitro for 24 h, and then inseminated in vitro for 12 h. After insemination, the oocytes were cultured in five media [Ham's F10, Waymouth 752/1 (Waymouth), TCM199, modified Earle's balanced salt solution (MK-1) and CR1aa], each of which contained 0.4… CONTINUE READING

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