Effects of selenoprotein S on oxidative injury in human endothelial cells

Abstract

Selenoprotein S (SelS) is an important endoplasmic reticulum and plasma membrane-located selenoprotein implicated in inflammatory responses and insulin resistance. However, the effects of SelS on endothelial cells (ECs) have not been reported. In the present study, the role of SelS in oxidative stress and the underlying mechanism were investigated in human ECs. A SelS over-expression plasmid (pc-SelS) and a SelS-siRNA plasmid were transfected into human umbilical vein endothelial cells (American Type Culture Collection, USA). The cells were divided into four groups: control, SelS over-expression (transfected with pc-SelS), vector control, and SelS knockdown (transfected with siRNA-SelS). After treating the cells with H2O2, the effects of oxidative stress and the expression of caveolin-1 (Cav-1) and protein kinase Cα (PKCα) were investigated. Following treatment with H2O2, over-expression of SelS significantly increased cell viability and superoxide dismutase (SOD) activity, and decreased malondialdehyde (MDA) production and Cav-1 gene and protein expression. However, no effects on PKCα were observed. In contrast, knockdown of SelS significantly decreased cell viability, SOD activity, and PKCα gene and protein expression, and increased MDA production and Cav-1 gene and protein expression. SelS protects ECs from oxidative stress by inhibiting the expression of Cav-1 and PKCα.

DOI: 10.1186/1479-5876-11-287

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@inproceedings{Zhao2013EffectsOS, title={Effects of selenoprotein S on oxidative injury in human endothelial cells}, author={Yin Zhao and Hua Li and Li-li Men and Rong-chong Huang and Hai-cheng Zhou and Qian Xing and Jun-jie Yao and Chun-hong Shi and Jian-Ling Du}, booktitle={Journal of Translational Medicine}, year={2013} }