Effects of retinoids on glycosaminoglycan synthesis by human skin fibroblasts grown as monolayers and within contracted collagen lattices

@article{Edward1995EffectsOR,
  title={Effects of retinoids on glycosaminoglycan synthesis by human skin fibroblasts grown as monolayers and within contracted collagen lattices},
  author={Michael Edward},
  journal={British Journal of Dermatology},
  year={1995},
  volume={133}
}
  • M. Edward
  • Published 1995
  • Chemistry, Medicine
  • British Journal of Dermatology
Fibroblasts grown within contracted collagen lattices synthesize substantially less glycosaminoglycans than fibroblasts grown as monolayers on a plastic substrate. [3H]glucosamine incorporation into hyaluronate was reduced by 70%, and incorporation into sulphated glycosaminoglycans was reduced by 40%. However, incorporation into heparan sulphate and chondroitin sulphates was reduced by 14 and 49%, respectively, resulting in a substantial change in the proportions of the individual… Expand
Modulation of sulfated glycosaminoglycan and small proteoglycan synthesis by the extracellular matrix
TLDR
An up regulation of biglycan mRNA steady state level and down regulation of decorin mRNA was observed, in comparison to monolayers, indicating that collagen can modulate the phenotypical expression of small proteoglycan genes. Expand
Differential effect of etretinate on proliferation and extracellular matrix metabolism of human dermal fibroblasts from elderly and young individuals in a novel three-dimensional culture.
TLDR
The results suggest that etretinate may have the differential effect on collagen and GAG metabolisms depending on the donor age of the cultured fibroblasts. Expand
Tumour regulation of fibroblast hyaluronan expression: a mechanism to facilitate tumour growth and invasion.
TLDR
In summary, the C8161 melanoma cells stimulated the synthesis of fibroblast hyaluronan, and in collagen lattices, only the invasive tumour cells expressed CD44 and hyaluranan, either in the presence or absence of Fibroblasts. Expand
Melanoma cell‐derived factors stimulate glycosaminoglycan synthesis by fibroblasts cultured as monolayers and within contracted collagen lattices
  • M. Edward
  • Biology, Medicine
  • The British journal of dermatology
  • 2001
TLDR
This work has shown that glycosaminoglycan rich matrices surrounding tumours that facilitate tumour growth and invasion are predominantly synthesized by fibroblasts following stimulation by tumour cell‐derived factors. Expand
Retinoic Acid, GABA-ergic, and TGF-β Signaling Systems Are Involved in Human Cleft Palate Fibroblast Phenotype
TLDR
It is demonstrated for the first time that GABA receptor (GABRB3) mRNA expression was upregulated in human CLP-SP fibroblasts, suggesting that altered crosstalk between RA, GABAergic, and TGF-β signaling systems could be involved in human cleft palate fibroblast phenotype. Expand
Hyaluronan in skin: aspects of aging and its pharmacologic modulation.
TLDR
High molecular size reflects intact tissues and antiangiogenic and immunosuppressive state, whereas smaller polymers are distress signals and potent inducers of inflammation and angiogenesis. Expand
Keratinocyte regulation of TGF‐β and connective tissue growth factor expression: A role in suppression of scar tissue formation
  • S. B. Amjad, R. Carachi, M. Edward
  • Chemistry, Medicine
  • Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
  • 2007
TLDR
It is suggested that keratinocytes may affect the TGF‐β profile in such a way as to suppress the formation of scar tissue. Expand
Three-dimensional organotypic matrices from alternative collagen sources as pre-clinical models for cell biology
TLDR
A step-by-step process to isolate collagen I from commercially available animal byproducts is demonstrated and both collagen sources showed equal applicability for invasive, proliferative or survival assessment of well-established cancer models and clinically relevant patient-derived cancer cell lines. Expand
Retinoids in Tumor Cell Adhesion, Invasion, and Metastasis
TLDR
The effects of retinoids on the various steps involved in the development and progression of a tumor from in situ to invasive to metastatic lesions are focused upon. Expand
4‐Methylumbelliferone inhibits tumour cell growth and the activation of stromal hyaluronan synthesis by melanoma cell‐derived factors
TLDR
Inhibition of tumour stimulation of fibroblast hyaluronan may suppress tumour growth and invasion. Expand
...
1
2
...

References

SHOWING 1-10 OF 40 REFERENCES
Synthesis of glycosaminoglycans by human skin fibroblasts cultured on collagen gels.
TLDR
Comparison has been made of the synthesis of glycosaminoglycans by human skin fibroblasts cultured on plastic or collagen gel substrata, and cells on collagen showed a 3-fold increase in dermatan sulphate synthesis, which could be due to a positive-feedback mechanism activated by the accumulation of dermatan sulfate in the microenvironment of the cultured cells. Expand
Retinoic acid inhibition of hyaluronate synthesis in cultured human skin fibroblasts.
  • T. Smith
  • Biology, Medicine
  • The Journal of clinical endocrinology and metabolism
  • 1990
TLDR
The effects of all-trans retinoic acid on glycosaminoglycan (GAG) accumulation were determined in cultured primary human skin fibroblasts and a pulse-chase study failed to demonstrate any effect on [3H]GAG degradation. Expand
Specific association of iduronic acid-rich dermatan sulphate with the extracellular matrix of human skin fibroblasts cultured on collagen gels.
TLDR
The patterns of synthesis and distribution of sulphated glycosaminoglycans observed in skin fibroblasts maintained on collagen gels may reflect differentiated cellular functions. Expand
The effect of cell density on net rates of glycosaminoglycan synthesis and secretion by cultured rat fibroblasts.
Net rates of synthesis and secretion into the medium for hyaluronic acid and chondroitin sulfate have been calculated at varying cell densities of fibroblastic cell cultures from several tissues ofExpand
Procollagen I synthesis in human skin fibroblasts: effect on culture conditions on biosynthesis.
TLDR
Collagenase digestion was used to study overall collagen biosynthesis and under optimal conditions, collagen comprised 24% of all protein in the medium, and 14% of protein produced by the whole culture. Expand
All-trans retinoic acid stimulates growth and extracellular matrix production in growth-inhibited cultured human skin fibroblasts.
TLDR
The data indicate that all-trans retinoic acid can induce changes in dermal fibroblasts in vitro (i.e., increased proliferation and extracellular matrix production) that mimic the major changes seen in the dermis after topical treatment with this agent. Expand
Influence of retinoic acid and TGF-beta on dermal fibroblast proliferation and collagen production in monolayer cultures and dermal equivalents.
TLDR
The data indicate that the extracellular matrix strongly influences the actions of growth factors and of RA on dermal fibroblasts. Expand
Collagen biosynthesis by cells in a tissue equivalent matrix in vitro.
TLDR
Cells grown in tissue lattices exhibit much higher collagenolytic activity than cells in monolayer suggesting the model may also be of special use for studying collagen turnover and matrix remodeling. Expand
The synthesis of a family of structurally related proteoglycans in fibroblasts is differently regulated by TFG-beta.
TLDR
The regulatory effect of transforming growth factor-beta, interleukin-1 (IL-1) platelet-derived growth factor (PDGF) and dexamethasone was determined by studies of mRNA levels for these structurally related proteoglycans, and the synthesis and secretion of these macromolecules was studied using radioactive precursors. Expand
Growth rate of human fibroblasts is repressed by the culture within reconstituted collagen matrix but not by the culture on the matrix.
TLDR
The results suggest that the repression of cell growth by collagen fibrils in the three-dimensional gels is not due to direct cell-cell contact, but due to the distribution and number of contact sites between a cell and collagenfibrils. Expand
...
1
2
3
4
...