Effects of repeated subculturing and prolonged storage at room temperature of enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles.

@article{Iguchi2002EffectsOR,
  title={Effects of repeated subculturing and prolonged storage at room temperature of enterohemorrhagic Escherichia coli O157:H7 on pulsed-field gel electrophoresis profiles.},
  author={Atsushi Iguchi and Ro Osawa and Junichi Kawano and Akira Shimizu and Jun Terajima and Haruo Watanabe},
  journal={Journal of clinical microbiology},
  year={2002},
  volume={40 8},
  pages={3079-81}
}
Three clinical strains of enterohemorrhagic Escherichia coli O157:H7 which were subcultured repeatedly or stored at room temperature over a 25-week period showed appreciable variations in their pulsed-field gel electrophoresis fragment patterns. The variations could be explained by a couple of spontaneous genetic events at most and thus did not invalidate the genetic lineage of the strains. 

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