Effects of reduced muscle glycogen on excitation–contraction coupling in rat fast-twitch muscle: a glycogen removal study

@article{Watanabe2019EffectsOR,
  title={Effects of reduced muscle glycogen on excitation–contraction coupling in rat fast-twitch muscle: a glycogen removal study},
  author={Daiki Watanabe and M. Wada},
  journal={Journal of Muscle Research and Cell Motility},
  year={2019},
  pages={1-12}
}
  • Daiki Watanabe, M. Wada
  • Published 2019
  • Biology, Medicine
  • Journal of Muscle Research and Cell Motility
  • The aim of this study was to investigate the effects of an enzymatic removal of glycogen on excitation–contraction coupling in mechanically skinned fibres of rat fast-twitch muscles, with a focus on the changes in the function of Na+–K+-pump and ryanodine receptor (RyR). Glycogen present in the skinned fibres and binding to microsomes was removed using glucoamylase (GA). Exposure of whole muscle to 20 U mL−1 GA for 6 min resulted in a 72% decrease in the glycogen content. Six minutes of GA… CONTINUE READING

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