Effects of pyruvate decarboxylase overproduction on flux distribution at the pyruvate branch point in Saccharomyces cerevisiae.

@article{Hoek1998EffectsOP,
  title={Effects of pyruvate decarboxylase overproduction on flux distribution at the pyruvate branch point in Saccharomyces cerevisiae.},
  author={Paul H W van Hoek and Marcel T. Flikweert and Q J van der Aart and Herman Steensma and Johannes P van Dijken and Jack T. Pronk},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 6},
  pages={2133-40}
}
A multicopy plasmid carrying the PDC1 gene (encoding pyruvate decarboxylase; Pdc) was introduced in Saccharomyces cerevisiae CEN. PK113-5D. The physiology of the resulting prototrophic strain was compared with that of the isogenic prototrophic strain CEN.PK113-7D and an empty-vector reference strain. In glucose-grown shake-flask cultures, the introduction of the PDC1 plasmid caused a threefold increase in the Pdc level. In aerobic glucose-limited chemostat cultures growing at a dilution rate of… CONTINUE READING