Effects of pressure and temperature on the binding of RecA protein to single-stranded DNA.

Abstract

The binding and polymerization of RecA protein to DNA is required for recombination, which is an essential function of life. We study the pressure and temperature dependence of RecA binding to single-stranded DNA in the presence of adenosine 5'-[γ-thio]triphosphate (ATP[γ-S]), in a temperature regulated high pressure cell using fluorescence anisotropy… (More)
DOI: 10.1073/pnas.1112646108

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