Effects of preservation and storage of microcrustaceans in RNA later on RNA and DNA degradation

  title={Effects of preservation and storage of microcrustaceans in RNA later on RNA and DNA degradation},
  author={Elena Gorokhova},
Methods of preserving nucleic acids are increasingly in demand because of the recent advances in molecular and biochemical approaches to ecology. The RNA storage reagent RNAlater® was tested as an alternative method to deep-freezing for preserving RNA and DNA in zooplankton. Artemia spp. nauplii were used as test organisms. Individual RNA and DNA contents were monitored over a time period of 8 months to evaluate the effects of preservation and storage. Treatments included (1) freezing at –80°C… CONTINUE READING
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Relationships between nucleic acid levels and egg production rates in Acartia bifilosa: implications for growth assessment of copepods in the northern Baltic proper

  • E. Gorokhova
  • Mar. Ecol. Prog. Ser. 262:163-172.
  • 2003
Highly Influential
5 Excerpts

Effects of freeze-thaw stress during soil storage on microbial communities and methidathion degradation

  • M. Pesaro, F. Widmer, G. Nicollier, J. Zeyer.
  • Soil Biol. Biochem. 35:1049-1061.
  • 2003
2 Excerpts

Interannual variation in phytoplankton blooms and zooplankton productivity and abundance in the Gulf of Maine during winter

  • E. G. Durbin, R. G. Campbell, +4 authors M. Wagner.
  • Mar. Ecol. Prog. Ser. 254:81-100.
  • 2003
2 Excerpts

The influence of food quality (P:C) ratio on RNA:DNA ratio and somatic growth rate of Daphnia

  • T. Vrede, J. Persson, G. Aronsen.
  • Limnol. Oceanogr. 47:487-494. Wagner, M., E…
  • 2002
2 Excerpts

Evidence for food limitation of Calanus finmarchicus production rates on the southern flank of Georges Bank during April 1997

  • R. G. Campbell, J. A. Runge, E. G. Durbin.
  • Deep-Sea Res. II 48:531-549.
  • 2001
2 Excerpts

Nucleic acids and growth of Calanus finmarchicus in the laboratory under different food and temperature conditions

  • R. G. Campbell, C. A. Boudreau, E. G. Durbin
  • 2001
1 Excerpt

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