Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.

  title={Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.},
  author={Ibrahim Abdullah Alsarra and Steven Henry Neau and Matthew A. Howard},
  volume={25 13},
The influences of the pH, tripolyphosphate (TPP) concentration, and ionic strength of the gelling medium on the entrapment efficiency, release, and activity of lipase in chitosan hydrogel beads were studied. A solution of Candida rugosa lipase was prepared in a 1.5% w/v chitosan and 1% (v/v) acetic acid medium, and dropped into a TPP solution. Release of lipase in pH 7.2 Tris buffer was monitored over 36 h using the micro BCA protein assay. The activity of the entrapped enzyme was assayed using… CONTINUE READING


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