Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.

@article{Alsarra2004EffectsOP,
  title={Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.},
  author={Ibrahim Abdullah Alsarra and Steven Henry Neau and Matthew A. Howard},
  journal={Biomaterials},
  year={2004},
  volume={25 13},
  pages={2645-55}
}
The influences of the pH, tripolyphosphate (TPP) concentration, and ionic strength of the gelling medium on the entrapment efficiency, release, and activity of lipase in chitosan hydrogel beads were studied. A solution of Candida rugosa lipase was prepared in a 1.5% w/v chitosan and 1% (v/v) acetic acid medium, and dropped into a TPP solution. Release of lipase in pH 7.2 Tris buffer was monitored over 36 h using the micro BCA protein assay. The activity of the entrapped enzyme was assayed using… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 34 references

Measurement of protein using bicinchoninic acid.

Analytical biochemistry • 1985
View 3 Excerpts
Highly Influenced

A specific method for serum lipase determination.

Clinica chimica acta; international journal of clinical chemistry • 1966
View 3 Excerpts
Highly Influenced

Lipase immobilization onto polymeric membranes

MG Carneiro-da-Cunha, JM Rocha, FA Garcia, MH. Gil
Biotechnol Tech • 1999

Characterization of microbial endo-b-glucanase immobilized in alginate beads

MD Busto, N Ortega, M. Perez-Mateos
Acta Biotechnol • 1998
View 1 Excerpt

Protein loss by the microencapsulation of an enzyme (lactase) in alginate beads

A. Dashevsky
Int J Pharm • 1998
View 1 Excerpt

Use of natural polysaccharides in microencapsulation techniques

E. Murano
J Appl Ichthyol • 1998
View 1 Excerpt

Production of biotech compounds

S. Kadir
1997
View 1 Excerpt

Effect of molecular weight of chitosan with the same degree of deacetylation on the thermal, mechanical, and permeability properties of the prepared membrane

RH Chen, HD Hwa
Carbohydr Polym 1996;29:253–60 • 1996
View 1 Excerpt

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