Effects of plasmid amplification and recombinant gene expression on the growth kinetics of recombinant E. coli.

@article{Betenbaugh1989EffectsOP,
  title={Effects of plasmid amplification and recombinant gene expression on the growth kinetics of recombinant E. coli.},
  author={Michael J. Betenbaugh and C D Beaty and Prasad Dhurjati},
  journal={Biotechnology and bioengineering},
  year={1989},
  volume={33 11},
  pages={1425-36}
}
An experimental study was undertaken to identify and quantitate the effects of plasmid amplification and recombinant gene expression on Escherichia coli growth kinetics. Identification of these effects was possible because recombinant gene expression and plasmid copy number were controlled by different mechanisms on plasmid pVH106/172. Recombinant gene expression of the lactose operon structural genes was under the control of the lac promoter and was activated by the addition of the chemicals… CONTINUE READING

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