Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells.

@article{Nakatani1984EffectsOP,
  title={Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells.},
  author={Yoshihito Nakatani and M Tsunoi and Yoshiyuki Hakeda and Noriyoshi Kurihara and Kiyohide Fujita and Masayoshi Kumegawa},
  journal={Biochemical and biophysical research communications},
  year={1984},
  volume={123 3},
  pages={
          894-8
        }
}
The effect of parathyroid hormone (PTH) was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 0.3% bovine serum albumin. PTH stimulated an increase in cAMP production in a dose-related fashion up to a concentration of 0.5 unit/ml, the maximum increase being 23-fold above that of controls. Although PTH did not affect DNA content, this peptide also increased dose-dependently alkaline phosphatase activity up to a concentration of 0.5 unit/ml. PTH plus… CONTINUE READING

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