Effects of parathyroid hormone on cAMP production and alkaline phosphatase activity in osteoblastic clone MC3T3-E1 cells.


The effect of parathyroid hormone (PTH) was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 0.3% bovine serum albumin. PTH stimulated an increase in cAMP production in a dose-related fashion up to a concentration of 0.5 unit/ml, the maximum increase being 23-fold above that of controls. Although PTH did not affect DNA… (More)


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