Effects of pH on in vitro ovulation of goldfish (Carassius auratus) oocytes.

  title={Effects of pH on in vitro ovulation of goldfish (Carassius auratus) oocytes.},
  author={Frederick William Goetz and Yoshitaka Nagahama},
  journal={The Journal of experimental zoology},
  volume={235 1},
The in vitro effects of pH on goldfish (Carassius auratus) ovulation were investigated. Final oocyte maturation and follicular detachment were induced in vivo in gravid goldfish by HCG injections and elevated holding temperatures. Females were biopsied to determine the time of germinal vesicle breakdown and when oocytes should be removed for in vitro incubations. Prior to ovulation, the ovaries were removed, dissected and mature intrafollicular oocytes were incubated in media of varying pH (7.3… CONTINUE READING

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