Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle.

@article{Orisaka2006EffectsOO,
  title={Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle.},
  author={Makoto Orisaka and Tetsuya Mizutani and Kimihisa Tajima and Sanae Orisaka and Ken-ichi Shukunami and Kaoru Maebashi-shi Miyamoto and Fumikazu Kotsuji},
  journal={Molecular reproduction and development},
  year={2006},
  volume={73 6},
  pages={
          737-44
        }
}
We investigated the effects of theca cells or FSH on granulosa cell differentiation and steroid production during bovine early follicular growth, using a co-culture system in which granulosa and theca cells were cultured on opposite sides of a collagen membrane. Follicular cells were isolated from early antral follicles (2-4 mm) that were assumed to be in gonadotropin-independent phase and just before recruitment into a follicular wave. Granulosa cells were cultured under serum-free conditions… CONTINUE READING
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