Effects of ovarian steroids on the gonadotropin response to N-methyl-D-aspartate and on hypothalamic excitatory amino acid levels during sexual maturation in female rats.

  title={Effects of ovarian steroids on the gonadotropin response to N-methyl-D-aspartate and on hypothalamic excitatory amino acid levels during sexual maturation in female rats.},
  author={Silvia Carbone and Berta Szwarcfarb and Matilde E. Otero Losada and Jaime A. Moguilevsky},
  volume={130 3},
In order to evaluate the involvement of estrogen-progesterone (EP) in the effects of N-methyl-D-aspartate (NMDA) receptor stimulation on gonadotropin secretion during sexual development in female rats, NMDA (30 mg/kg sc) was administered to 16- and 30-day-old female rats pretreated with EP. NMDA administration induced increases in plasma LH concentration that were 13.6-fold and 94.5-fold higher, respectively, than those found after NMDA alone. The increase of LH levels induced by NMDA was… 
N-methyl-D-aspartic acid receptor messenger ribonucleic acid levels and luteinizing hormone release in immature female rats: effects of stage of pubertal development and exposure to ethanol.
Findings demonstrate regional differences in the timing of NMda-R gene expression in the reproductive hypothalamus during pubertal development, show differential responses of LH to NMDA-R activation during the peripubertal period, and continue to demonstrate the vulnerability of the hypothalamic-pituitary axis to the detrimental effects of ETOH at this critical time of development.
Role of excitatory amino acid pathways in control of gonadotrophin secretion in adult female rats sterilized by neonatal administration of oestradiol or testosterone.
The results obtained in sterilized females showed both a tonic release of endogenous excitatory amino acids and a greater responsiveness to NMDA and kainic acid than in controls.
Glutamate: A major neuroendocrine excitatory signal mediating steroid effects on gonadotropin secretion
  • D. BrannV. Mahesh
  • Biology, Medicine
    The Journal of Steroid Biochemistry and Molecular Biology
  • 1995
Regulation of luteinizing hormone-releasing hormone (LHRH) secretion by melatonin in the ewe. II. Changes in N-methyl-D,L-aspartic acid-induced LHRH release during the stimulation of luteinizing hormone secretion by melatonin.
The purpose of the present experiment was to determine whether this effect of photoperiod on LH response toNMDA is mediated by melatonin and whether it reflects a change in the amount of LHRH released in response to NMDA.
Sexual differences in the role of kainate receptors in controlling gonadotrophin secretion in prepubertal rats.
The results indicate that the role of kainate receptors in the control of gonadotrophin secretion is sexually dimorphic around puberty, since: (a) LH secretion was stimulated by kainic acid in male rats but inhibited in females; (b) FSH secretion was inhibited by kainsate acid in ovariectomized females, but not in orchidECTomized males; and (c) DNQX inhibited LH secretion in males but notIn females.


A role for N-methyl-D-aspartate (NMDA) receptors in the control of LH secretion and initiation of female puberty.
It is demonstrated that blockade of NMDA receptors can prevent the development of enhanced LH secretion in female rats undergoing sexual maturation, and this results support the view that activation ofNMDA receptors significantly contributes to the physiological initiation of the pubertal process.
Endogenous excitatory amino acid involvement in the preovulatory and steroid-induced surge of gonadotropins in the female rat.
Findings demonstrate that the preovulatory gonadotropin surge in the female rat is dependent on NMDA neurotransmission for its expression and add further evidence for a critically important role for NMDA receptors in the physiological regulation of gonadotropic secretion in theFemale rat.
Endogenous excitatory amino acid neurotransmission regulates the estradiol-induced LH surge in ovariectomized rats.
Results indicate that endogenous EAA play an important role in controlling LH secretion and reveals that both EAA receptor types, NMDA and non-NMDA, appear to be necessary for the physiological mechanism(s) triggering the estradiol-induced LH surge.
The effects of neurally active amino acids on pituitary gonadotropin secretion.
Evidence is provided for a possible role for certain neurally excitant amino acids in the neuronal control of LH secretion and a significant increase in LH levels following injection into the pituitary of anesthetized male rats.
Puberty in monkeys is triggered by chemical stimulation of the hypothalamus.
It is reported that prolonged intermittent NMDA stimulation of GnRH neurons within the hypothalamus of the juvenile monkey for 16-30 wk results, with surprising ease, in the onset of precocious puberty with full activation of the hypothalamic-pituitary-Leydig cell axis and initiation of spermatogenesis.
Activation of luteinizing hormone-releasing hormone release advances the onset of female puberty.
The juvenile-peripubertal transition period in the female rat is associated with an ovarian-independent afternoon increase in the amplitude of plasma luteinizing hormone (LH) pulses, and the LHRH neuronal system can be precociously activated by the episodic administration of an excitatory amino acid analog that is known to interact with specific brain receptors.
Sheep stalk-median eminence extract was subjected to gel filtration and it was concluded that sheep hypothalamic extract contains discrete FSH- and LH-releasing factors.
A specific and very sensitive assay method for FSH using intact immature female mice was devised. This method is based on the synergism between FSH and a small amount of human chorionic gonadotrophin
Parameters of electrical stimulation of the medial preoptic area for release of gonadotrophins in male rats.
The significance of the importance of the medial preoptic area in the male rat, the neurones which may be involved in the regulation of gonadotrophin secretion, and the parameters of stimulation used in studying the hypothalamo-hypophysial system are discussed.