Effects of organic pH buffers on a cell growth and an antibody production of human-human hybridoma HB4C5 cells in a serum-free culture

@article{Nagira2004EffectsOO,
  title={Effects of organic pH buffers on a cell growth and an antibody production of human-human hybridoma HB4C5 cells in a serum-free culture},
  author={K. Nagira and M. Hayashida and M. Shiga and K. Sasamoto and K. Kina and K. Osada and T. Sugahara and H. Murakami},
  journal={Cytotechnology},
  year={2004},
  volume={17},
  pages={117-125}
}
  • K. Nagira, M. Hayashida, +5 authors H. Murakami
  • Published 2004
  • Biology, Medicine
  • Cytotechnology
  • Human-human hybridoma cells secreting a human monoclonal antibody were cultured in a serum-free medium containing various organic pH buffers in order to clarify their effects on cell growth and antibody production. Organic pH buffers having either one sulfonic acid and several acyclic amine moieties, or several cyclic amine moieties containing two amino nitrogen did not inhibit cell growth; while other organic buffers sulfonic acid moiety plus several cyclic amine moieties containing one amino… CONTINUE READING
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