Effects of organelle shape on fluorescence recovery after photobleaching.

@article{Sbalzarini2005EffectsOO,
  title={Effects of organelle shape on fluorescence recovery after photobleaching.},
  author={Ivo F. Sbalzarini and Anna Mezzacasa and Ari Helenius and Petros Koumoutsakos},
  journal={Biophysical journal},
  year={2005},
  volume={89 3},
  pages={1482-92}
}
The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods. Fluorescence recovery after photobleaching (FRAP) experiments are performed in tissue culture cells expressing GFP-KDEL, a soluble, fluorescent protein, in the ER… CONTINUE READING

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Multiscale flow simulations using particles

  • P. Koumoutsakos
  • Annu. Rev. Fluid Mech. 37:457–487
  • 2005
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