Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.

@article{Raeder2007EffectsOO,
  title={Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium.},
  author={Sten Raeder and Tor Paaske Utheim and O A Utheim and Bj{\o}rn Nicolaissen and Borghild Roald and Yiqing Cai and Kristiane Haug and Anders Kvalheim and Edvard Berger Messelt and Liv K Drolsum and John C. Reed and Torstein Lyberg},
  journal={Investigative ophthalmology & visual science},
  year={2007},
  volume={48 12},
  pages={
          5484-93
        }
}
PURPOSE A previous report has described the use of eye bank storage of cultured human limbal epithelial cells (HLECs) to provide a reliable source of tissue for treating limbal stem cell deficiency. In the present study, conventional organ culture (OC) storage and Optisol-GS (Bausch & Lomb, Irvine, CA) storage of cultured HLECs were compared. METHODS Three-week HLEC cultures were either organ cultured at 31 degrees C or 23 degrees C or stored in Optisol-GS at 5 degrees C in a closed container… CONTINUE READING

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