Effects of octyl beta-glucoside on insulin binding to solubilized membrane receptors.

@article{Gould1981EffectsOO,
  title={Effects of octyl beta-glucoside on insulin binding to solubilized membrane receptors.},
  author={Robert J. Gould and B H Ginsberg and Arthur A. Spector},
  journal={Biochemistry},
  year={1981},
  volume={20 24},
  pages={6776-81}
}
Octyl beta-glucoside (1%), a dialyzable detergent, was used to solubilize the insulin receptor of the turkey erythrocyte membrane. Insulin binding capacity was stable for at least 1 week when the receptor was kept in 1% octyl beta-glucoside at 4 degrees C. The binding properties of the solubilized receptor were examined at detergent concentrations above (1%) and below (0.6%) the critical micelle concentration. A reduction in insulin binding occurred when the detergent concentration was raised… CONTINUE READING

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