Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease.

@article{Inouye2007EffectsON,
  title={Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease.},
  author={K. Inouye and T. Shimada and K. Yasukawa},
  journal={Journal of biochemistry},
  year={2007},
  volume={142 3},
  pages={
          317-24
        }
}
Streptomyces caespitosus neutral protease (ScNP) is one of the smallest metalloproteinase with a molecular mass of 14 kDa. Effects of solvent composition on ScNP activity were examined using a peptide substrate. The k(cat)/K(m) values of ScNP exhibited bell-shaped pH-dependence with the optimal pH of 6.4-7.0 and the pK(a) values of 5.0 +/- 0.1 and 8.3 +/- 0.1. ScNP activity increased in an exponential fashion with increasing [NaCl]. The relative k(cat)/K(m) value at 3.6 M NaCl to that at 0 M… Expand

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