Effects of mutations on the thermodynamics of a protein folding reaction: implications for the mechanism of formation of the intermediate and transition states.

@article{Lorch2000EffectsOM,
  title={Effects of mutations on the thermodynamics of a protein folding reaction: implications for the mechanism of formation of the intermediate and transition states.},
  author={M. J. Lorch and Jody M Mason and Richard B. Sessions and A. R. Clarke},
  journal={Biochemistry},
  year={2000},
  volume={39 12},
  pages={3480-5}
}
We have measured changes in heat capacity, entropy, and enthalpy for each step in the folding reaction of CD2.d1 and evaluated the effects of core mutations on these properties. All wild-type and mutant forms fold through a rapidly formed intermediate state that precedes the rate-limiting transition state. Mutations have a pronounced effect on the enthalpy of both the intermediate and folded states, but in all cases a compensatory change in entropy results in a small net free-energy change… CONTINUE READING