Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage.

@article{Burns1989EffectsOM,
  title={Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage.},
  author={Cara Burns and Mark A. Lawson and Bert L Semler and Ellie Ehrenfeld},
  journal={Journal of virology},
  year={1989},
  volume={63 11},
  pages={4866-74}
}
A series of short insertion mutations was introduced into the poliovirus gene for 3Dpol at a number of different locations. When substituted for wild-type sequences in a full-length, infectious cDNA and tested for infectivity, all 3D mutants were nonviable. The mutant cDNAs were introduced into a bacterial plasmid designed to direct the expression of poliovirus 3CD, a viral protein composed of contiguous protease and RNA polymerase sequences. Bacteria transformed with these plasmids all… CONTINUE READING