Effects of mutations in Aedes aegypti sterol carrier protein-2 on the biological function of the protein.

@article{Radek2010EffectsOM,
  title={Effects of mutations in Aedes aegypti sterol carrier protein-2 on the biological function of the protein.},
  author={James T. Radek and David H. Dyer and Que Lan},
  journal={Biochemistry},
  year={2010},
  volume={49 35},
  pages={7532-41}
}
Sterol carrier protein-2 (SCP-2) is a nonspecific intracellular lipid carrier protein. However, the molecular mechanism of ligand selectivity and the in vivo function of SCP-2 remain unclear. In this study, we used site-directed mutagenesis to investigate the ligand selectivity and in vivo function of the yellow fever mosquito sterol carrier protein-2 protein (AeSCP-2). Mutations to amino acids in AeSCP-2 known to interact with bound ligand also weakened NBD-cholesterol binding. Substitution of… CONTINUE READING