Effects of motexafin gadolinium on DNA damage and X-ray-induced DNA damage repair, as assessed by the Comet assay.

  title={Effects of motexafin gadolinium on DNA damage and X-ray-induced DNA damage repair, as assessed by the Comet assay.},
  author={Erling T. Donnelly and Yanfeng Liu and Tracy K Paul and Sara Rockwell},
  journal={International journal of radiation oncology, biology, physics},
  volume={62 4},
PURPOSE To investigate the effects of motexafin gadolinium (MGd) on the levels of reactive oxygen species (ROS), glutathione (GSH), and DNA damage in EMT6 mouse mammary carcinoma cells. The ability of MGd to alter radiosensitivity and to inhibit DNA damage repair after X-ray irradiation was also evaluated. METHODS AND MATERIALS Reactive oxygen species and GSH levels were assessed by 2,7-dichlorofluorescein fluorescence flow cytometry and the Tietze method, respectively. Cellular… Expand
10 Citations
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Chemical radiosensitizers for use in radiotherapy.
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  • Medicine
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Patterns of Persistent DNA Damage Associated with Sun Exposure and the Glutathione S‐transferase M1 Genotype in Melanoma Patients
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Combinations of Ionizing Radiation and Other Sensitizing Agents
Two specific agents, recently used as “radiation enhancers,” are addressed in the present chapter, including the redox modulator motexafin-gadolinium (MGd), and the alkylating agent temozolomide, a DNA alkylator, which are the focus of this chapter. Expand
Phase I trial of motexafin gadolinium and doxorubicin in the treatment of advanced malignancies
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Lanthanides: Applications in Cancer Diagnosis and Therapy.
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Should concomitant and adjuvant treatment with temozolomide be used as standard therapy in patients with anaplastic glioma?
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Gd-Tex sensitizes cells to ionizing radiation by increasing oxidative stress as a consequence of futile redox cycling and optimization of the concentration of ascorbate (or other reducing species) may be required when evaluating Gd- Tex activity in vitro. Expand
Effects of glutathione depletion by buthionine sulfoximine on the sensitivity of EMT6/SF cells to chemotherapy agents or X radiation.
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Effects of RSR13 and oxygen on the cytotoxicity of cisplatin and carboplatin to EMT6 mouse mammary tumor cells in vitro and in vivo
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Motexafin gadolinium: a redox active drug that enhances the efficacy of bleomycin and doxorubicin.
  • R. Miller, K. Woodburn, +5 authors D. Magda
  • Medicine, Biology
  • Clinical cancer research : an official journal of the American Association for Cancer Research
  • 2001
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Role of glutathione in the radiation response of mammalian cells in vitro and in vivo.
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The role of thiols in cellular response to radiation and drugs.
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The interacting pathways for prevention and repair of oxidative DNA damage.
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A simple technique for quantitation of low levels of DNA damage in individual cells.
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A modified neutral comet assay: elimination of lysis at high temperature and validation of the assay with anti-single-stranded DNA antibody.
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