• Biology
  • Published 2003

Effects of minor arginyl tRNA and isoleucyl tRNA on the expression of Clostridium botulinum neurotoxin light chain in Escherichia coli

@inproceedings{Kim2003EffectsOM,
  title={Effects of minor arginyl tRNA and isoleucyl tRNA on the expression of Clostridium botulinum neurotoxin light chain in Escherichia coli},
  author={Jin Sook Kim and Hye Young Seong and Mi Wha Kim and Jong Seo Ku and Soon-Yong Choi},
  year={2003}
}
Botulinum neurotoxin type A (BONT/A) is an extremely potent toxin, which is produced by Clostridium botulinum. The light chain of this protein (BONT/A LC), which is known as a zinc endopeptidase, cleaves SNAP-25 involved in the exocytosis process. In this work, the expression of recombinant BoNT/A LC in E. coli is described. The BONT/A LC gene of C. botulinum contains a high frequency of the arginine AGA and isoleucine ATA codons that are rarely used in genes of E. coli, hampering the… CONTINUE READING