Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea

@article{Reeves2006EffectsOM,
  title={Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea},
  author={Andrew R. Reeves and Igor A. Brikun and William H. Cernota and Benjamin I. Leach and Melissa C. Gonzalez and J. Mark Weber},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={2006},
  volume={33},
  pages={600-609}
}
In carbohydrate-based fermentations of Saccharopolyspora erythraea, a polar knockout of the methylmalonyl-CoA mutase (MCM) gene, mutB, improved erythromycin production an average of 126% (within the range of 102–153% for a 0.95 confidence interval). In oil-based fermentations, where erythromycin production by the wild-type strain averages 184% higher (141–236%, 0.95 CI) than in carbohydrate-based fermentations, the same polar knockout in mutB surprisingly reduced erythromycin production by 66… 
Engineering of the methylmalonyl-CoA metabolite node for increased erythromycin production in oil-based fermentations of Saccharopolyspora erythraea
TLDR
The combined results showed that increased erythromycin production only occurred in strain FL2385 containing a duplication of the entire MCM operon including mutR and a predicted stem-loop structure overlapping the 3′ terminus of the mutR coding sequence.
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TLDR
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TLDR
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TLDR
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TLDR
This study showed that isoflavone metabolism could be blocked in A. erythreum by ery BI knockout but that eryBI knockout was not sufficient to block is oflav one metabolism in S. eries, suggesting that other β-glucosidases are present.
PccD Regulates Branched-Chain Amino Acid Degradation and Exerts a Negative Effect on Erythromycin Production in Saccharopolyspora erythraea
TLDR
The results demonstrated that PccD controlled the supply of precursors for biosynthesis of erythromycin via regulating the BCAA degradation and propionyl-CoA assimilation and exerted a negative effect on erystromycin production.
A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253
TLDR
The results demonstrated that the combined approach involving traditional mutagenesis and metabolic engineering could be successfully applied to the diagnosis of yield-limiting factors and the enhanced production of industrially and clinically important polyketide compounds.
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