Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges.

@article{Mizuno2001EffectsOM,
  title={Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges.},
  author={Shuichi Mizuno and Florin Allemann and Julie Glowacki},
  journal={Journal of biomedical materials research},
  year={2001},
  volume={56 3},
  pages={368-75}
}
Various culture systems have been used for examining the anabolic and catabolic functions of isolated chondrocytes as well as for tissue engineering purposes. Perfusion or frequent medium change is beneficial for three-dimensional (3D) cultures of many cell types. In this study, bovine articular chondrocytes (bACs) were grown in 3D collagen sponges with or without medium perfusion (0.33 mL/min) for up to 15 days. The influence of medium perfusion was evaluated using markers of cartilage matrix… CONTINUE READING

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