Effects of magnesium on the kinetic properties of bovine heart glycogen synthase D.

@article{Nakai1975EffectsOM,
  title={Effects of magnesium on the kinetic properties of bovine heart glycogen synthase D.},
  author={Chiharu Nakai and Jim A. Thomas},
  journal={The Journal of biological chemistry},
  year={1975},
  volume={250 11},
  pages={
          4081-6
        }
}
Highly purified glycogen synthase D, free of synthase kinase and phosphatase activities, was prepared from bovine heart. The enzyme had no activity without glucose 6-phosphate. Kinetic studies of this enzyme at various concentrations of UDP-glucose demonstrated that there was no cooperativity with respect to the substrate at any concentration of glucose-6-P with or without Mg2+. Glucose 6-phosphate increased the maximum velocity (Vmax) of the enzyme, but had very little or no effect on the… CONTINUE READING

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