Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro.

Abstract

This investigation was performed to determine the effect of lipopolysaccharide (LPS) on production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. After confluence, the cells were stimulated with LPS (0.1, 1.0 or 10 micrograms/ml) for 4, 8, 24, and 48 hr. LPS increased production of both IL-1 and IL-6 production from mammary cells in a dose dependent manner. The expression of mRNA for IL-1 receptor antagonist (IL-1ra) was demonstrated by reverse transcription-polymerase chain reaction in bovine mammary epithelial cells.

Cite this paper

@article{Okada1999EffectsOL, title={Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro.}, author={Hiroshi Okada and Hiromichi Ohtsuka and S Kon Nai and Rikio Kirisawa and Yuichi Yokomizo and Tadashi Yoshino and Thomas J Rosol}, journal={The Journal of veterinary medical science}, year={1999}, volume={61 1}, pages={33-5} }