Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells.

Abstract

This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 microM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 microM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor (TNF)-alpha tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.

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Cite this paper

@article{Okada1999EffectsOI, title={Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells.}, author={Hiroshi Okada and Y I Miyake and Hiromichi Ohtsuka and Yoshio Kiku and Satoshi Fukuda and Akiharu Watanabe and Yuichi Yokomizo and Thomas J Rosol and Tadashi Yoshino}, journal={The Journal of veterinary medical science}, year={1999}, volume={61 5}, pages={553-6} }