Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts.

@article{Currie1987EffectsOI,
  title={Effects of ischemia and perfusion temperature on the synthesis of stress-induced (heat shock) proteins in isolated and perfused rat hearts.},
  author={R. William Currie},
  journal={Journal of molecular and cellular cardiology},
  year={1987},
  volume={19 8},
  pages={795-808}
}
Isolated and perfused rat hearts were examined by 2-dimensional gel electrophoresis and liquid scintillation counting to determine the effect of ischemia or perfusion temperature on protein synthesis. Isolated hearts were subjected to ischemia at either 4 degrees, 20 degrees, or 30 degrees C and then perfused at 37 degrees C and radio-labeled with [3H]-leucine from 0.5 to 2.5 h of perfusion. Following 0.5 h of 4 degrees C or 0.2 h of 20 degrees C ischemia, minimal or no effect was seen by… CONTINUE READING