Effects of interleukins 1, 2 and 3 on follicle-stimulating hormone-induced differentiation of rat granulosa cells

  title={Effects of interleukins 1, 2 and 3 on follicle-stimulating hormone-induced differentiation of rat granulosa cells},
  author={Barry G. Kasson and William C. Gorospe},
  journal={Molecular and Cellular Endocrinology},
  • B. Kasson, W. Gorospe
  • Published 1 March 1989
  • Biology, Medicine
  • Molecular and Cellular Endocrinology
Interleukin-1beta inhibits steroidogenic bioactivity in cultured rat ovarian granulosa cells by stimulation of progesterone degradation and inhibition of estrogen formation.
An IL-1beta-mediated inhibition of gonadotropin-stimulated steroidogenesis via modulation of specific enzymes is demonstrated, and a role for IL- 1beta is suggested in mediating the observed decline of these bioactive hormones during ovulation and luteolysis.
Interleukin-6: effects on and production by rat granulosa cells in vitro.
The results of the present study suggest a role for IL-6 in the regulation of progesterone production, that the granulosa cell is a source of IL- 6 and that the release ofIL-6 by the Granulosa Cell is a regulated event.
Interleukin-2 is a potent inhibitor of Leydig cell steroidogenesis.
The results suggest that a T cell growth factor, IL-2, is a potent inhibitor of steroidogenesis and may play a paracrine role in modulating Leydig cell function.
Effects of interleukin-6 on proliferation and follicle-stimulating hormone-induced estradiol production by bovine granulosa cells in vitro: dependence on size of follicle.
IL-6 has a more potent inhibitory effect on proliferation and FSH-induced estradiol production by bovine granulosa cells collected from small follicles than from large follicles, and it appears that less differentiated granULosa cells (small follicles) are more sensitive to IL-6 than are highly differentiated granul Rosa cells (large follicles).
Inhibition by gonadotropins of interleukin-1 production by rabbit granulosa and theca cells: effects on gonadotropin-induced progesterone production.
Gonadotropins, by inhibiting IL-1 production, could control IL- 1 modulation of gonadotropin action on steroidogenesis in a dose-dependent manner, and it is concluded that in this model gonadotropic action on steroidsogenesis could be controlled.
Interleukin-6 production by rat granulosa cells in vitro: effects of cytokines, follicle-stimulating hormone, and cyclic 3',5'-adenosine monophosphate.
Results show that the granulosa cell is not only a likely source ofIL-6 but that the release of IL-6 can be regulated, and evidence suggests that cAMP may serve as a second messenger for the stimulated secretion of Il-6 by undifferentiatedgranulosa cells.
Effects of Bacterial Endotoxin (Lipopolysaccharide) on FSH-Induced Granulosa Cell Activities
The preliminary results have shown that bacterial endotoxin (lipopolysaccharide, LPS) exerted inhibitory effects on FSH-induced formation of LH receptors in rat GCs, suggesting the possibility that LPS may act either directly on GCs or may act indirectly by inducing the production of a secondary factor that influences GC function.
Cytokine-mediated regulation of ovarian function: interleukin-1 inhibits gonadotropin-induced androgen biosynthesis.
Findings indicate that relatively low concentrations of IL-1, possibly originating from somatic ovarian cells or resident ovarian macrophages, are capable of exerting an inhibitory effect upon gonadotropin-supported androgen production.
Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro.
It is concluded that human recombinant IL-2 significantly inhibits progesterone production stimulated by hCG in human granulosa-luteal cells and had a marked inhibitory effect on forskolin-induced progestersone release.
Lipopolysaccharide inhibits in vitro luteinizing hormone-stimulated rat ovarian granulosa cell estradiol but not progesterone secretion.
The results indicate that LPS inhibits in vitro GC estradiol secretion, and this effect appears to be restricted to the LH-stimulated aromatization of androgens to estrogen and may involve a tyrosine kinase signaling pathway.


Interleukin 1: an inhibitor of luteinizing hormone receptor formation in cultured rat granulosa cells
It is suggested that IL 1 decreases the number of LH receptors and that protein synthesis may be necessary for IL 1's action, however, a physiological/pathological role for IL 2 in ovarian regulation has yet to be established.
Interleukin-1 inhibits luteinization of porcine granulosa cells in culture.
It was shown for the first time that IL-1 can modulate steroidogenic functions of mammalian cells in culture and markedly inhibited the LH-stimulated progesterone production in a dose-dependent manner.
Lymphokines from concanavalin-A-stimulated lymphocytes regulate rat granulosa cell steroidogenesis in vitro.
The present studies demonstrate that secretory products of lymphocytes (collectively termed lymphokines) can affect steroidogenesis in cultured rat granulosa cells, implying that immune cell factors may play a significant role in the differentiation and maturation of granULosa cells.
Homologous regulation of hormone receptors: luteinizing hormone increases its own receptors in cultured rat granulosa cells.
LH, like FSH, is capable of stimulating granulosa cell differentiation by inducing its own receptors, which serves as an interesting model for studies on the positive autoregulation of hormone receptors and explains the important role of LH during advanced stages of follicular maturation.
Both interleukin 2 and a second T cell-derived factor in EL-4 supernatant have activity as differentiation factors in IgM synthesis
Results indicate that IL-2 binds to a receptor on appropriately prepared B cells and causes them to differentiate into high rate IgM-synthesizing cells, and the physiologic significance of the B cell differentiative activity ofIL-2 remains to be investigated.
Synergism between granulosa and theca-interstitial cells in estrogen biosynthesis by gonadotropin-treated rat ovaries: studies on the two-cell, two-gonadotropin hypothesis using steroid antisera.
Combined cell cultures showed synergistic interactions between granulosa and theca interstitial cells are important in effecting maximal estrogen biosynthesis, indicating synergism between the two types of cell.
Interleukin 2 and a lactogen regulate proliferation and protein phosphorylation in Nb2 cells.
The results suggest that hGH and IL-2 act through separate receptors to stimulate proliferation of Nb2 cells, and that some of the actions of both mitogens may be mediated, in part, through regulation of protein phosphorylation.