• Biology, Medicine
  • Published in
    The Journal of biological…
    1992

Effects of insulin on steady state kinetics of GLUT4 subcellular distribution in rat adipocytes. Evidence of constitutive GLUT4 recycling.

@article{Jhun1992EffectsOI,
  title={Effects of insulin on steady state kinetics of GLUT4 subcellular distribution in rat adipocytes. Evidence of constitutive GLUT4 recycling.},
  author={Byung H Jhun and Amrit Rampal and Hongtu Liu and Mohsen Lachaal and Chan Young Jung},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 25},
  pages={
          17710-5
        }
}
We labeled rat adipocyte cell surface glucose transporters with an impermeable, photoreactive glucose analogue, 1,3-bis-(3-deoxy-D-glucopyranose-3-yloxy)-2-propyl 4-benzoylbenzoate (B3GL) and its radioactive tracer [3H]B3GL. The labeling did not affect glucose transporter subcellular distribution in basal and insulin-stimulated adipocytes. When basal or insulin-stimulated adipocytes were labeled with [3H]B3GL and incubated at 37 degrees C in steady state, labeled GLUT4 was rapidly reduced at… CONTINUE READING

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