Effects of hypoxia on stimulus-release coupling mechanisms in cultured bovine adrenal chromaffin cells

Abstract

To clarify the effects of hypoxia on stimulus-release coupling, we have examined the effects of hypoxia on nicotine-induced catecholamine (noradrenaline and adrenaline) release from, and 22Na+ influx, 45Ca2+ influx and cytosolic free Ca2+ concentration ([Ca2+]i) in, cultured bovine adrenal chromaffin cells. Experiments were carried out in media pre-equilibrated with 21% O2/79% N2 (control) or with 0% O2/100% N2 (hypoxia). Cells were stimulated with either nicotine (activating nicotinic acetylcholine (ACh) receptors) or a high K+ concentration (55 mmol/1 KCI; directly activating voltage-dependent Ca2+ channels). Hypoxia reduced both nicotine- and high K+-induced catecholamine releases from the cells, but the reduction of the former (to about 30% of the control value) was more pronounced than that of the latter (to about 40% of the control value). Nicotine-induced 22Na+ influx, which is considered to reflect the function of nicotinic ACh receptors, was inhibited by hypoxia. Both nicotine- and high K+-induced 45Ca2+ influx into the cells were reduced by hypoxia, but the reduction of the former was more pronounced than that of the latter. Nicotine- and high K+-induced increases in [Ca2+]i were reduced by hypoxia to about 30% and 40% of the control values, respectively. These results suggest that hypoxia reduces cation influxes (Na+ and Ca2+) through both the ligand-gated cation channels of the nicotinic ACh receptor and the voltage-dependent Ca2+ channels.

DOI: 10.1007/BF00169156

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@article{Lee1993EffectsOH, title={Effects of hypoxia on stimulus-release coupling mechanisms in cultured bovine adrenal chromaffin cells}, author={Ken Wing Kuen Lee and Akihiro Sekine}, journal={Naunyn-Schmiedeberg's Archives of Pharmacology}, year={1993}, volume={348}, pages={275-281} }