Effects of hygromycin on cotton cultures and its application in Agrobacterium-mediated cotton transformation

@article{Meng2007EffectsOH,
  title={Effects of hygromycin on cotton cultures and its application in Agrobacterium-mediated cotton transformation},
  author={Zhao-Hong Meng and Ai-hua Liang and Wei-Cai Yang},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={43},
  pages={111-118}
}
We have evaluated the effects of the antibiotic hygromycin B on cotton (Gossypium hirsutum L.) callus induction, callus proliferation, and seed germination. Nontransgenic cotyledon and hypocotyl showed obvious variance in tolerance to hygromycin. Cotyledons were more sensitive to hygromycin than hypocotyls. Hygromycin at 7.5 and 20 mg l−1 completely inhibited callus initiation from cotyledon and hypocotyl explants, respectively. Nontransformed calli did not grow on media supplemented with 10 mg… CONTINUE READING
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