Effects of homocysteine on number and activity of endothelial progenitor cells from peripheral blood.

@article{Chen2004EffectsOH,
  title={Effects of homocysteine on number and activity of endothelial progenitor cells from peripheral blood.},
  author={Jian Zhong Chen and Jia Hao Zhu and Xiao Xiong Wang and Xun De Xie and Joyce Sun and Yunpeng P Shang and Xiao Guo Guo and Helena M Dai and Shze Jer Hu},
  journal={Journal of molecular and cellular cardiology},
  year={2004},
  volume={36 2},
  pages={
          233-9
        }
}
The aim of this study is to investigate whether homocysteine (Hcy) has influences on endothelial progenitor cells (EPCs) number and activity. Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture dishes. After 7 d cultured, attached cells were stimulated with Hcy (to make a series of final concentrations: 10, 50, 100 and 200 micromol/l) or vehicle control for the respective time… CONTINUE READING
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