Effects of heptaminol AMP amidate (HAA) on cyclic AMP level and mitogen-induced proliferation of murine spleen cells.

  title={Effects of heptaminol AMP amidate (HAA) on cyclic AMP level and mitogen-induced proliferation of murine spleen cells.},
  author={K. Ueno and A. Saha and H. Kitagawa and T. Satoh},
  journal={Japanese journal of pharmacology},
  volume={52 3},
Heptaminol AMP amidate (HAA), a nucleotide derivative, was found to elevate the intracellular cyclic AMP (cAMP) level of cultured mice spleen cells in a time- and dose-dependent manner. Theophylline and imidazole, when added to the spleen cell culture simultaneously with HAA, respectively caused a further rise and a fall of the cAMP level increased by HAA alone. When comparatively higher doses of the T cell mitogen concanavalin A (Con A) were used in the culture, Con A-induced cell… Expand


Effect of heptaminol AMP amidate, a new nucleotide derivative, on in vitro humoral immunity.
The present results suggested that HAA-mediated immunopotentiation might be in some way related to the intracellular level of cyclic nucleotides in the early phase of culture. Expand
Effects of heptaminol AMP amidate on suppressor and helper function of murine T cells.
The present results suggested that HAA-mediated immunopotentiation was possible by a combined suppressive effect on Ts cells and enhancing effect on Th cells. Expand
The antagonistic action of cyclic GMP and cyclic AMP on proliferation of B and T lymphocytes.
The effect of c-AMP, c-GMP and both substances together on (3H)-thymidine incorporation into nuclear DNA was investigated using spleen cells of normal and athymic nude mice and the possible role of cyclic nucleotides in the regulation of cell proliferation and of the immune response is discussed. Expand
Role of cyclic adenosine 3',5'-monophosphate in lymphocyte mitogenesis.
It seems possible that elevated cellular cAMP levels may only delay, by a reversible process, the flow of mitogen-activated lymphocytes through their growth cycle and that lymphocytes remain susceptible to such inhibition for only a finite period after stimulation with mitogens. Expand
Immunopotentiating activity of a nucleotide derivative, heptaminol AMP amidate (HAA) in mice and spontaneously hypertensive rats (SHR).
In spontaneously hypertensive rats, with a state of immunosuppression, 10 days consecutive administrations of HAA at the dose of 10 mg/kg was found to increase significantly the anti-SRBC PFC and antibody titer values. Expand
Biphasic effect of cyclic AMP on an immune response
The same agents which might enhance an AFC response if present during the increased cyclic AMP phase, could inhibit the response ifPresent during the subsequent phase of declining intracellular cyclicAMP. Expand
Guanosine 3':5'-cyclic monophosphate: a possible intracellular mediator of mitogenic influences in lymphocytes.
A revised hypothesis is presented in which a temporally discrete rise in lymphocyte cyclic GMP concentration is presented as an active signal to induce proliferation, while the elevation of cyclic AMP concentration in these cells is viewed as a regulatory influence that limits or inhibits mitogenic action. Expand
Cyclic AMP and immune responses: changes in the splenic level of cyclic AMP during the response of mice to antigen.
Data provide direct evidence for a role of cAMP as a mediator in the activation and proliferation of immunocytes stimulated by antigen, and one can avoid indiscriminate use of drugs that modify the level of endogenous cAMP and instead employ them rationally in controlling the immune response, enhancing or suppressing it as desired. Expand
Selective induction of DNA synthesis in T and B lymphocytes.
It is suggested that Con A-activated T cells can influence B cells to respond to Con A; whereas B cells by themselves cannot be activated by Con A, suggesting that LPS exerts a non-specific stimulatory effect on B cells. Expand
Characterization of a specific adenosine receptor on human lymphocytes.
The results suggest that adenosine increases the intracellular cAMP content of lymphocytes as a result of its interaction with a specific membrane receptor which results in the activation of adenylate cyclase. Expand