Effects of heparin on polymerase chain reaction for blood white cells.

@article{Yokota1999EffectsOH,
  title={Effects of heparin on polymerase chain reaction for blood white cells.},
  author={Mitsuhiro Yokota and Noriyuki Tatsumi and Oytip Nathalang and Teri Yamada and Izumi Tsuda},
  journal={Journal of clinical laboratory analysis},
  year={1999},
  volume={13 3},
  pages={133-40}
}
Polymerase chain reaction (PCR) has been used with increasing frequency to diagnose infectious and genetic diseases. In this study, the effects of heparin on PCR were investigated, because heparinized blood may sometimes be used in PCR studies. HLA-DQA1 gene amplification was used as a model. PCR was clearly interfered with when heparinized blood was used as a source of template DNA, and the degree of interference was affected by the following three factors; (1) type of Taq DNA polymerase; (2… CONTINUE READING