Effects of hemin and thermal stress exposure on JWA expression.

  title={Effects of hemin and thermal stress exposure on JWA expression.},
  author={Ming Xu Zhao and Rui Chen and Aiping Li and Jianwei Zhou},
  journal={Frontiers of medicine in China},
  volume={1 1},
To investigate the expression of JWA after hemin and (or) thermal stress exposure, we treated K562 (chronic myelogenous leukemia cells) cells with different doses of hemin and thermal stress using different exposure times. The expression of JWA protein was determined by Western blot analysis. Reverse transcription-polymerase chain reaction was carried out to determine JWA mRNA expression. JWA promoter transcription activity analysis was performed by chloramphenicol acetyl transferase-enzyme… CONTINUE READING

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