Effects of guanine nucleotide and sulfhydryl reagent on subpopulations of muscarinic acetylcholine receptors in mammalian hearts: possible evidence for interconversion of super-high and low affinity agonist binding sites.

@article{Uchida1984EffectsOG,
  title={Effects of guanine nucleotide and sulfhydryl reagent on subpopulations of muscarinic acetylcholine receptors in mammalian hearts: possible evidence for interconversion of super-high and low affinity agonist binding sites.},
  author={Shinya Uchida and Kiyoshi Matsumoto and Akiko Mizushima and Tomoaki Osugi and Hiroshi Higuchi and Hiroshi Yoshida},
  journal={European journal of pharmacology},
  year={1984},
  volume={100 3-4},
  pages={291-8}
}
Multiple site models of muscarinic acetylcholine receptors (mAChR) for agonist binding were applied to curves for the inhibition of QNB binding by carbachol by using nonlinear least square regression analysis. The effects of a guanine nucleotide guanyl-5'-yl imidodiphosphate (Gpp(NH)p) and a sulfhydryl reagent 5,5'-dithiobis(2-nitrobenzoic acid (DTNB) on the curves were also analyzed. The results suggested that mAChR of dog and guinea pig heart had three types of sites with different affinities… CONTINUE READING