Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture.

@article{Vriezen1997EffectsOG,
  title={Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture.},
  author={Nienke Vriezen and B. Romein and K C Luyben and Johannes P. van Dijken},
  journal={Biotechnology and bioengineering},
  year={1997},
  volume={54 3},
  pages={
          272-86
        }
}
  • Nienke Vriezen, B. Romein, +1 author Johannes P. van Dijken
  • Published in
    Biotechnology and…
    1997
  • Chemistry, Medicine
  • Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology of two mammalian cell lines were studied in steady-state chemostat cultures fed with IMDM medium with 5% serum. The cell lines used were MN12, a mouse-mouse hybridoma, and SP2/0-Ag14, a mouse myeloma often used in hybridoma… CONTINUE READING

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