Effects of glutamine, glycine and taurine on the development of in vitro fertilized bovine zygotes in a chemically defined medium.

@article{Takahashi1998EffectsOG,
  title={Effects of glutamine, glycine and taurine on the development of in vitro fertilized bovine zygotes in a chemically defined medium.},
  author={Yumi Takahashi and Hiroshi Yamakawa Kanagawa},
  journal={The Journal of veterinary medical science},
  year={1998},
  volume={60 4},
  pages={433-7}
}
The effects of glutamine, glycine and taurine on the development of bovine zygotes derived from IVM/IVF were determined using a protein-free chemically defined medium. After the cumulus cells were removed at 18 hr post-insemination, the presumptive zygotes were cultured for 4 or 6 days (about 104 or 154 hr) under a gas atmosphere of 5% O2: 5% CO2: 90% N2. A modified synthetic oviduct fluid medium supplemented with 20 amino acids (1 mM glutamine, essential amino acids for basal medium Eagle and… CONTINUE READING