Effects of global or targeted deletion of the EP4 receptor on the response of osteoblasts to prostaglandin in vitro and on bone histomorphometry in aged mice.

@article{Gao2009EffectsOG,
  title={Effects of global or targeted deletion of the EP4 receptor on the response of osteoblasts to prostaglandin in vitro and on bone histomorphometry in aged mice.},
  author={Qi Gao and Peili Zhan and Cynthia B Alander and Barbara E. Kream and Chuanming Hao and Matthew D. Breyer and Carol C. Pilbeam and Lawrence G. Raisz},
  journal={Bone},
  year={2009},
  volume={45 1},
  pages={98-103}
}
Because global deletion of the prostaglandin EP4 receptor results in neonatal lethality, we generated a mouse with targeted EP4 receptor deletion using Cre-LoxP methodology and a 2.3 kb collagen I a1 promoter driving Cre recombinase that is selective for osteoblastic cells. We compared wild type (WT), global heterozygote (G-HET), targeted heterozygote (T-HET) and knockout (KO) mice. KO mice had one targeted and one global deletion of the EP4 receptor. All mice were in a mixed background of… CONTINUE READING