Effects of ginkgo biloba on in vitro osteoblast cells and ovariectomized rat osteoclast cells

  title={Effects of ginkgo biloba on in vitro osteoblast cells and ovariectomized rat osteoclast cells},
  author={Seung Min Oh and Ha Ryong Kim and Kyu Hyuck Chung},
  journal={Archives of Pharmacal Research},
Ginkgo biloba extract (GBE) has a selective estrogen receptor modulator (SERM)-like biphasic effect on estrogen, and could be a potential alternative to hormone replacement therapy (HRT). Here, we investigated whether GBE can ameliorate estrogen-depleted osteoporosis in in vitro osteoblast cells and in estrogen-deprived ovariectomized (OVX) rats, a classical animal model for postmenopausal osteoporosis. GBE (50–150 μg/mL) significantly increased ALP (Alkaline phosphatase) activity of osteoblast… Expand
Ginkgo biloba extract (EGb761) did not express estrogenic activity in an immature rat uterotrophic assay
Evaluated agonistic and antagonistic activities of EGb761 using a uterotrophic assay in immature female rats suggested EGb 761 has no agonistic/antagonistic effects in utero. Expand
The Effect of the Ginkgo biloba Extract in the Expression of Bax, Bcl‐2 and Bone Mineral Content of Wistar Rats with Glucocorticoid‐Induced Osteoporosis
The EGb improved the Bcl‐2 and reduced the Bax expression by osteoblasts in the mandibular alveolar bone and recovered the mineral content in the tibia of rats with glucocorticoid‐induced‐osteoporosis. Expand
Simvastatin induces estrogen receptor-alpha expression in bone, restores bone loss, and decreases ERα expression and uterine wet weight in ovariectomized rats
It is suggested that simvastatin exert opposing modulatory effects on ERα expression on bone and uterus in ovariectomized rats, inducing ER α expression and synergy with estrogen to perform anabolic effects on the bones while decreasing E2 efficacy and uterine wet weight. Expand
The Inhibitory Effects of the Standardized Extracts of Ginkgo biloba on Aromatase Activity in JEG‐3 Human Choriocarcinoma Cells
Ginkgo biloba could act as an AI at both the enzyme and transcriptional levels, and aromatase activities were inhibited by EGb both in JEG‐3 cells and in recombinant CYP19 microsomes. Expand
Ginkgo biloba extract EGb 761-mediated inhibition of aromatase for the treatment of hormone-dependent breast cancer.
  • Y. Park, H. Ahn, Ha Ryong Kim, K. Chung, S. Oh
  • Chemistry, Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2016
Results indicated that EGb 761 inhibited aromatase and exerted antitumor effects on breast cancer cells both in vitro and in–vivo, and suggest that EG b761 may be a useful aromat enzyme inhibitor for the treatment for estrogen-sensitive breast cancer. Expand
Evaluation of the anti-osteoporotic effect of Ginkgo biloba L. in Wistar rats with glucocorticoid-induced-osteoporosis by bone densitometry using dual-energy x-ray absorptiometry (DEXA) and mechanical testing.
The extract of Ginkgo biloba restored bone alkaline phosphatase and bone mineral density using dual-energy x-ray absorptiometry and restored the mechanical properties of the tibia in rats with glucocorticoid-induced-osteoporosis. Expand
Inhibitory Aromatase Effects of Flavonoids from Ginkgo Biloba Extracts on Estrogen Biosynthesis.
It is demonstrated that three flavonoid synergistically inhibit estrogen biosynthesis through aromatase inhibition, decrease CYP19 mRNA, and induce transcriptional suppression, which support the usefulness of flavonoids in adjuvant therapy for breast cancer by reducing estrogen levels with reduced adverse effects due to estrogen depletion. Expand
The effects of the standardized extracts of Ginkgo biloba on steroidogenesis pathways and aromatase activity in H295R human adrenocortical carcinoma cells
It is suggested that EGb761 could regulate steroidogenesis-related genes such as CYP19 and 17 β-HSD1, and lead to a decrease in 17β-estradiol and testosterone. Expand
Radiographic evidence of mandibular osteoporosis improvement in Wistar rats treated with Ginkgo biloba
The EGb recovered the periodontal bone support and increased the mandibular cortical thickness, suggesting that the EGb may be effective in the treatment of osteoporosis. Expand
Effects of Flavonoids from Gingko Biloba Extracts on Estrogen Biosynthesis
Since estrogen is a key hormone that stimulates cancer growth during the initiation and promotion stages, regulating estrogen is important in the treatment of hormone-dependent breast cancer (YagerExpand


The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro
HEP treatment can effectively suppress the OVX-induced increase in bone turnover possibly by both an increase in osteoblastic activities and a decrease in osteoclastogenesis. Expand
Daidzein is more efficient than genistein in preventing ovariectomy-induced bone loss in rats.
Consumption of 17 alpha-ethinylestradiol or daidzein was more efficient than genistein in preventing ovariectomy-induced bone loss in rats, a model for postmenopausal osteoporosis. Expand
The Synthetic Phytoestrogen, Ipriflavone, and Estrogen Prevent Bone Loss by Different Mechanisms
Findings indicate that IP is effective in preventing the ovx-associated bone loss and the bone protective mechanisms of IP in ovarian hormone deficiency may be different from those of E2 and may involve increased rates of bone formation. Expand
The Phytoestrogen Genistein Reduces Bone Loss in Short-Term Ovariectomized Rats
This study shows that GEN reduces both trabecular and compact bone loss after ovariectomy and that this protective effect differs from that of estrogen, since it depends on stimulation of bone formation rather than on suppression of bone resorption. Expand
Effects of phytoestrogens and environmental estrogens on osteoblastic differentiation in MC3T3-E1 cells.
Phytoestrogens, such as coumestrol, genistein and daidzein, and BPA increased ALP activity and enhanced bone mineralization in MC3T3-E1 cells, suggesting that not only phy toestrogen but also BPA, an environmental estrogen, is implicated in bone metabolism. Expand
Preventive effects of the plant isoflavones, daidzin and genistin, on bone loss in ovariectomized rats fed a calcium-deficient diet.
The preventive effect of daidzin treatment on bone loss in ovariectomized rats appears to be due to suppression of bone turnover, which is similar to that seen in sham-operated rats. Expand
Antiestrogenic activities of Ginkgo biloba extracts
  • S. Oh, K. Chung
  • Medicine
  • The Journal of Steroid Biochemistry and Molecular Biology
  • 2006
GBE has a biphasic effect on estrogen, and can be considered as a potential alternative to HRT with chemopreventive effects on breast cancer, however, further studies on animals and humans will be required. Expand
Bone-Protecting Effect of Safflower Seeds in Ovariectomized Rats
The present data provide the first direct in vivo evidence that safflower seeds have a protecting effect on bone loss caused by estrogen deficiency, without substantial effect on the uterus. Expand
Estrogenic activities of Ginkgo biloba extracts.
The results indicated that GBE and its major components had the weak estrogenic activities through the estrogen response pathway by an interaction with the ER, which confirms the capacity as an alternative HRP. Expand
The protective effects of Ginkgo biloba extract (EGb 761) against free radical damage to osteoblast-like bone cells (MC3T3-E1) and the proliferative effects of EGb 761 on these cells
The antioxidative effects of EGb 761 against free radical damage showed a small but inconsistent protective effect, which may prove relevant to protecting against the loss of bone mass and the development of osteoporosis in human subjects. Expand